Neonatal sepsis is defined as a bacterial infection of the blood in an infant younger than 4 weeks of age. Blood cultures remain the mainstay of laboratory diagnosis of bloodstream infections (BSIs) in infants. The knowledge of bacteriological profile and its antibiotic sensitivity patterns is of immense help in saving lives of neonates with septicaemia. The study was conducted on neonatal blood samples to identify the common pathogens as well as rare and newer pathogenic species causing neonatal septicaemia along with their antibiotic sensitivity to help formulate guidelines for empiric antibiotic therapy in a developing country.