Bacterial infections caused by multidrug resistant bacteria (MDR) are a constant challenge for physicians throughout the world. We processed 451 clinical samples (blood, urine, pus and body fluids) from pediatric patients during the period of July’11 to Oct’11 in Sassoon General Hospital, Pune. Objective is to identify the bacterial isolates from different clinical specimens and study their antibiotics susceptibility pattern. Bacterial infections could be detected in 155 (34.4%) patients. These bacteria were isolated from blood (61%), urine (23%), pus (14%) and body fluid (2%). 64.6% of the infections were from the ward and 35.5% from NICU. 88.4% were gram negative bacilli and 11.7% were gram positive cocci.