This study therefore was carried out to determine the prevalence of Asymptomatic bacteriuria (ASB) in pregnant women and to isolate, identify the causative organisms; and to test the antimicrobial susceptibility of isolated pathogens. A total of 200 pregnant women who came for ante-natal checkup in outpatient department at Govt. Maternity Hospital, Nayapool. Hyderabad were studied over a period of one year.