Caries is a chronic disease that progresses slowly and is characterized by localized and irreversible destruction of the tooth. The microbial community of dental caries with many of the facultatively and obligately anaerobic bacteria, Lactobacillus have been consistently associated with dental caries for decades. The aim of this study was the isolation and characterization of Lactobacillus species, which are commonly present on the surface of dental caries of children.